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bcl 2β isoform  (Bio-Rad)


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    Bio-Rad bcl 2β isoform
    Bcl 2β Isoform, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/crithidia+luciliae+slides/Kallestad+Crithidia+luciliae+Slides+(20+x+8+Wells)/pmc12662848-158-18-21
    Average 90 stars, based on 2 article reviews
    bcl 2β isoform - by Bioz Stars, 2026-09
    90/100 stars

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    Incubation:

    Article Title: Dietary tryptophan and genetic susceptibility expand gut microbiota that promote systemic autoimmune activation
    Article Snippet: .. Anti-dsDNA IgG were also visualized on Crithidia luciliae slides (Bio-Rad) with serum diluted 1:10 followed by an incubation with goat anti-mouse IgG-FITC (1:100; Southern Biotech). .. Images were acquired on an inverted fluorescence microscope (Olympus).

    Article Title: Dietary tryptophan and genetic susceptibility expand gut microbiota that promote systemic autoimmune activation
    Article Snippet: .. Anti-dsDNA IgG were also visualized on Crithidia luciliae slides (Bio-Rad) with serum diluted 1:10 followed by an incubation with goat anti-mouse IgG-FITC (1:100; Southern Biotech). .. Images were acquired on an inverted fluorescence microscope (Olympus).

    Article Title: Gut Expansion of a Human Lupus Pathobiont is Associated With Autoantibody Production and T Cell Dysregulation
    Article Snippet: .. Anti‐dsDNA IgG was also visualized on Crithidia luciliae slides (Biorad) in serum diluted 1:10, followed by incubation with goat anti‐mouse IgG‐FITC (Southern Biotech). ..

    Article Title: Gut Expansion of a Human Lupus Pathobiont is Associated With Autoantibody Production and T Cell Dysregulation.
    Article Snippet: .. Serum and fecal anti-dsDNA antibodies were quantified by enzyme-linked immunosorbent assay (ELISA) with 1:100 dilutions, as previously described.9 Anti-dsDNA IgG was also visualized on Crithidia luciliae slides (Biorad) in serum diluted 1:10, followed by incubation with goat anti-mouse IgG-FITC (Southern Biotech). ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: Gut Expansion of a Human Lupus Pathobiont is Associated With Autoantibody Production and T Cell Dysregulation.
    Article Snippet: .. Serum and fecal anti-dsDNA antibodies were quantified by enzyme-linked immunosorbent assay (ELISA) with 1:100 dilutions, as previously described.9 Anti-dsDNA IgG was also visualized on Crithidia luciliae slides (Biorad) in serum diluted 1:10, followed by incubation with goat anti-mouse IgG-FITC (Southern Biotech). ..



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    The combination of gut microbiota from lupus‐prone mice and high dietary tryptophan promoted autoimmune activation. (A) Terminal serum anti‐dsDNA IgA and (B) total IgA. (C) Terminal cecal anti‐dsDNA IgA and (D) total IgA. (E) Representative images (scale bar: 20 μm) and quantitation of <t>Crithidia</t> <t>luciliae</t> assays on terminal serum. CTCF calculated on all visible IgG + cells. n = 3 to 9 FMT recipients per group. (F) Representative images (scale bar: 200 μm) and quantitation of CD3 + cell infiltrates in the kidney. All CD3 + cells were counted in the ×20 field. (G) Frequency of splenic B cells, plasmablasts, and pDCs. Frequency of mLN Treg cells in (H) CD4 + T cells and (I) IL‐10 + cells in Treg and non‐Treg CD4 + T cells. Mean + SEM compared with one‐way analysis of variance with multiple‐comparison tests. * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.001. anti‐dsDNA, anti–double‐stranded DNA; B6mbTrp, C57BL/6 microbiota fed tryptophan; CTCF, corrected total cell fluorescence; DAPI, 4',6‐diamidino‐2‐phenylindole; IL‐10, interleukin‐10; mLN, mesenteric lymph node; pDC, plasmacytoid dendritic cell; TCmbTrp, B6. Sle1.Sle2.Sle3 triple congenic microbiota fed tryptophan.
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    The combination of gut microbiota from lupus‐prone mice and high dietary tryptophan promoted autoimmune activation. (A) Terminal serum anti‐dsDNA IgA and (B) total IgA. (C) Terminal cecal anti‐dsDNA IgA and (D) total IgA. (E) Representative images (scale bar: 20 μm) and quantitation of <t>Crithidia</t> <t>luciliae</t> assays on terminal serum. CTCF calculated on all visible IgG + cells. n = 3 to 9 FMT recipients per group. (F) Representative images (scale bar: 200 μm) and quantitation of CD3 + cell infiltrates in the kidney. All CD3 + cells were counted in the ×20 field. (G) Frequency of splenic B cells, plasmablasts, and pDCs. Frequency of mLN Treg cells in (H) CD4 + T cells and (I) IL‐10 + cells in Treg and non‐Treg CD4 + T cells. Mean + SEM compared with one‐way analysis of variance with multiple‐comparison tests. * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.001. anti‐dsDNA, anti–double‐stranded DNA; B6mbTrp, C57BL/6 microbiota fed tryptophan; CTCF, corrected total cell fluorescence; DAPI, 4',6‐diamidino‐2‐phenylindole; IL‐10, interleukin‐10; mLN, mesenteric lymph node; pDC, plasmacytoid dendritic cell; TCmbTrp, B6. Sle1.Sle2.Sle3 triple congenic microbiota fed tryptophan.
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    The combination of gut microbiota from lupus‐prone mice and high dietary tryptophan promoted autoimmune activation. (A) Terminal serum anti‐dsDNA IgA and (B) total IgA. (C) Terminal cecal anti‐dsDNA IgA and (D) total IgA. (E) Representative images (scale bar: 20 μm) and quantitation of <t>Crithidia</t> <t>luciliae</t> assays on terminal serum. CTCF calculated on all visible IgG + cells. n = 3 to 9 FMT recipients per group. (F) Representative images (scale bar: 200 μm) and quantitation of CD3 + cell infiltrates in the kidney. All CD3 + cells were counted in the ×20 field. (G) Frequency of splenic B cells, plasmablasts, and pDCs. Frequency of mLN Treg cells in (H) CD4 + T cells and (I) IL‐10 + cells in Treg and non‐Treg CD4 + T cells. Mean + SEM compared with one‐way analysis of variance with multiple‐comparison tests. * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.001. anti‐dsDNA, anti–double‐stranded DNA; B6mbTrp, C57BL/6 microbiota fed tryptophan; CTCF, corrected total cell fluorescence; DAPI, 4',6‐diamidino‐2‐phenylindole; IL‐10, interleukin‐10; mLN, mesenteric lymph node; pDC, plasmacytoid dendritic cell; TCmbTrp, B6. Sle1.Sle2.Sle3 triple congenic microbiota fed tryptophan.
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    The combination of gut microbiota from lupus‐prone mice and high dietary tryptophan promoted autoimmune activation. (A) Terminal serum anti‐dsDNA IgA and (B) total IgA. (C) Terminal cecal anti‐dsDNA IgA and (D) total IgA. (E) Representative images (scale bar: 20 μm) and quantitation of Crithidia luciliae assays on terminal serum. CTCF calculated on all visible IgG + cells. n = 3 to 9 FMT recipients per group. (F) Representative images (scale bar: 200 μm) and quantitation of CD3 + cell infiltrates in the kidney. All CD3 + cells were counted in the ×20 field. (G) Frequency of splenic B cells, plasmablasts, and pDCs. Frequency of mLN Treg cells in (H) CD4 + T cells and (I) IL‐10 + cells in Treg and non‐Treg CD4 + T cells. Mean + SEM compared with one‐way analysis of variance with multiple‐comparison tests. * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.001. anti‐dsDNA, anti–double‐stranded DNA; B6mbTrp, C57BL/6 microbiota fed tryptophan; CTCF, corrected total cell fluorescence; DAPI, 4',6‐diamidino‐2‐phenylindole; IL‐10, interleukin‐10; mLN, mesenteric lymph node; pDC, plasmacytoid dendritic cell; TCmbTrp, B6. Sle1.Sle2.Sle3 triple congenic microbiota fed tryptophan.

    Journal: ACR Open Rheumatology

    Article Title: Gut Expansion of a Human Lupus Pathobiont is Associated With Autoantibody Production and T Cell Dysregulation

    doi: 10.1002/acr2.70033

    Figure Lengend Snippet: The combination of gut microbiota from lupus‐prone mice and high dietary tryptophan promoted autoimmune activation. (A) Terminal serum anti‐dsDNA IgA and (B) total IgA. (C) Terminal cecal anti‐dsDNA IgA and (D) total IgA. (E) Representative images (scale bar: 20 μm) and quantitation of Crithidia luciliae assays on terminal serum. CTCF calculated on all visible IgG + cells. n = 3 to 9 FMT recipients per group. (F) Representative images (scale bar: 200 μm) and quantitation of CD3 + cell infiltrates in the kidney. All CD3 + cells were counted in the ×20 field. (G) Frequency of splenic B cells, plasmablasts, and pDCs. Frequency of mLN Treg cells in (H) CD4 + T cells and (I) IL‐10 + cells in Treg and non‐Treg CD4 + T cells. Mean + SEM compared with one‐way analysis of variance with multiple‐comparison tests. * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.001. anti‐dsDNA, anti–double‐stranded DNA; B6mbTrp, C57BL/6 microbiota fed tryptophan; CTCF, corrected total cell fluorescence; DAPI, 4',6‐diamidino‐2‐phenylindole; IL‐10, interleukin‐10; mLN, mesenteric lymph node; pDC, plasmacytoid dendritic cell; TCmbTrp, B6. Sle1.Sle2.Sle3 triple congenic microbiota fed tryptophan.

    Article Snippet: Anti‐dsDNA IgG was also visualized on Crithidia luciliae slides (Biorad) in serum diluted 1:10, followed by incubation with goat anti‐mouse IgG‐FITC (Southern Biotech).

    Techniques: Activation Assay, Quantitation Assay, Comparison, Fluorescence